Cultivation of Mycobacterium leprae in Cell-Free, Semi-Synthetic Soft Agar Media

Cultivation and Viability Determination of Mycobacterium leprae

Mycobacterium leprae, despite being recognized as a human pathogen over 140 years ago, remains uncultivable in microbiological culture media or in cell culture systems. Although it is now well established that M. leprae prefers cooler temperatures, slightly acidic microaerophilic conditions, and lipids rather than sugars as an energy source, the exact parameters for a defined axenic medium that...

The mouse foot-pad technique for cultivation of Mycobacterium leprae.

Although multiplication of Mycobacterium leprae in the foot pads of immune-competent mice is limited, and no leprosy-like lesions are produced in these animals, the mouse foot-pad system represents the first truly useful and reproducible animal model of M. leprae infection. Its employment has enabled research into basic questions with respect to the microbiology of M. leprae, and the epidemiolo...

Attempts at cultivation of Mycobacterium leprae in cell culture under regulation of redox potential at environment.

Water Soluble Palmitic Acid in Media for Cultivation of Leprosy Derived Psychrophilic Mycobacteria from Mycobacterium Leprae Infected Tissues

A unique marker of Mycobacterium (M) leprae is that it cannot derive energy, use carbon sources, or substrates for biosynthetic purposes, whereas other mycobacteria utilize these for multiplication. This is just one reason why M. leprae, although provided with a complete Krebs cycle, a functional electron transport chain and cytochrome system, as well as measurable endogenous respiration, does ...

Demonstration of staphylococcal clumping factor and free coagulase in soft agar media.

Of the methods now available for the detection of coagulase positive staphylococei, none is designed to show both clumping factor and free coagulase activity in a single system. The observations reported here are by-products of a search for variant strains of staphylococci with particular reference to strain differences in the cell surface component known as clumping factor or bound coagulase. ...